Anti-diabetes activity of Kombucha prepared from different snake fruit cultivars

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Purpose: This paper aims to study the anti-diabetes activity of the Kombucha prepared from different snake fruit cultivars.Design/methodology/approach: The juices of snake fruits of Suwaru, Madura, Pondoh and Bali cultivars were fermented for 14 days. The anti-diabetes activity of the products was analyzed. Twenty-four male albino Wistar rats were used and randomly divided into six experimental groups, i.e., four groups of the diabetic rats treated with the Kombucha, plus the normal group and diabetic control group. The Kombucha was orally administered to the streptozotocin induced-diabetic rats at 5 mL/kg body weight per day during the 28-day experiment. The fasting plasma glucose (FPG), oxidative stress indices (superoxide dismutase [SOD] activity, and Malondialdehyde [MDA] level) and lipid profile of the blood plasma were measured. The pancreas was used for immunohistochemical study and b -cells quantification. Data were analyzed by ANOVA, followed by the Fisher test using Minitab version 16.0. Findings: FPG of the diabetic rats treated with the Kombucha (110.3-189.3 mg/dL) was significantly lower (p = 0.000) than the diabetic control group (413.3 mg/dL). Those were in line with the number of pancreatic b -cells of 42.1 in diabetic rats that lower (p = 006) than those in treated the diabetic rats (61.2-73.5). The treated diabetic rats had lower oxidative stress SOD activity: 20.9-44.6 unit/100 mL with p = 0.000; MDA level: 0.37-0.48 ng/100 mL with p = 0.000) than those in the diabetic rats (SOD activity: 18.7 unit/100mL; MDA level: 0.84 ng/100 mL). The treated diabetic rats also showed a better lipid profile than those in diabetic control rats. There were cultivar differences, and the Suwaru and Madura snake fruit Kombucha demonstrated the most potential for diabetes management. Originality/value: This is the first study on in vivo anti-diabetes activity of snake fruit Kombucha prepared from different snake fruit cultivars.

Keywords: cells, diabetes, glucose, rats, stress

Country: Indonesia, France

Citation: Nutrition & Food Science,

Study Mailing Address:
Elok Zubaidah and Raida Amelia Ifadah Department of Food Science and Technology, Brawijaya University, Malang, Indonesia,65145

Date Updated: March 25, 2020

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