Cytotoxic Activity Assay of N-Hexane Extract of Solanum nigrum L. Fruits Fermented by Kombucha against MCF-7 Breast Cancer Cell Line
Rahma Ziska, A Agustina, Dadih Supriadi
Background: Breast cancer is the second leading cause of cancer-related deaths among women worldwide. Anticancer drugs therapy causes unwanted side effects problems that occur when treatment affects healthy tissues or organs. It is quite essential to find potential herbal as an alternative cancer treatment. Glycoalkaloid (2-solanin), solamargin, solasodine, dan solasonin of Solanum nigrum L. are potentially inhibited proliferation of leukemia and tumor cell: hepatic, intestinal, breast and cervic. On the other hand, kombucha, as the symbiotic culture of bacteria and yeast (SCOBY), has the ability to increase the activity of substrate by fermentation process. Objective: To determine cytotoxicity activity of n-hexane fruits extract of Solanum nigrum L. against MCF-7 breast cancer cell line after fermented by kombucha. Method: n-hexane fruits extract of Solanum nigrum L. was fermented for eight days at 25 C with 20% of kombucha and addition of 20% of sucrose. Cytotoxic activity was performed by using MTT(3-(4,5-dimethyltiazole-2-yl)-2,5-diphenyltetrazolium bromide) assay. Phytochemical of n-hexane extract was analyzed by GC-MS. Total acid and total phenol were characterized quantitatively. Results: IC50 of n-hexane fruits extract, and fermentation product was 544,30 ppm and 1386,39 ppm, respectively. Flavonoids and steroids were identified from the extract, and GC-MS showed seven compounds had been extracted from n-hexane fraction. Conclusion: The condition of kombucha fermentation required optimization to improve the cytotoxic activity of n-hexane fruit extract of Solanum nigrum L. against the MCF-7 breast cancer cell line.
Keywords: cancer, ph, sucrose
Citation: Journal of Physics: Conf. Series 1338 , 012027
Study Mailing Address:
School of Pharmacy Bandung, Bandung, West Java, Indonesia
Date Updated: March 3, 2020