Effect of solvent fractions of kombucha tea on viability and invasiveness of cancer cells--Characterization of dimethyl 2-(2-hydoxy-2-ethoxypropylidine) malonate and vitexin
Rasu Jayabalan, Kesavan Malini, Muthuswamy Sathishkumar, Krishnaswami Swaminathan, Sei Eok Yun
In the present study, cytotoxic and anti-invasive properties of solvent fractions of kombucha tea were studied. Kombucha tea was fractionated with chloroform, ethyl acetate, and butanol. Three solvent extracts and the final aqueous phase were concentrated and used for anti-cancer study at 25, 50,75 and 100<mu>g/mL concentrations. Results revealed that ethyl acetate fraction at a concentration of 100 <mu>g/mL caused a cytotoxic effect on 786-O and U2OS cells; reduced the activities of matrix metalloproteinase-2 (MMP-2) and -9 in 786-O cells. Thus, ethyl acetate fraction was further purified by chromatographic studies, and the presence of dimethyl 2-(2-hydroxy-2-methoxypropylidene) malonate and vitexin were confirmed through IR, NMR and mass spectroscopic studies, which might be responsible for the observed anticancer property of kombucha tea.
Keywords: cancer, cells, ph, protein
Citation: This paper was presented in the International Conference on 'Challenges in Biotechnology and Food Technology' organized at Annamalai Universit,Annamalai Nagar
Study Mailing Address:
Dept of Biotechnology, Bharathiar University, Coimbatore 641 046, Indian
Date Updated: April 8, 2020