Examination of the Varied and Changing Ethanol Content of Commercial Kombucha Products

Authors:
Mohsen Talebi, Lillian A Frink, Rahul A Patel, Daniel W Armstrong

Abstract:
Kombucha is a fermented beverage made by mixing tea and sugar with bacteria and yeast. When kombucha products contain higher than 0.5% (v/v) alcohol, the legal limit for non-alcoholic drinks, they are classified as alcoholic beverages and are subject to relevant federal and state regulations. An efficient headspace gas chromatography technique utilizing an ionic liquid stationary phase is developed to accurately determine the ethanol content in 18 commercial kombucha samples. The range of ethanol in these products was 1.12-2.00% (v/v). The ethanol concentration of two batches of kombucha was analyzed over a period of 60 days under two different conditions. A significant increase in the ethanol content of these samples was observed at 4 and 22 C. The method accuracy was validated by analyzing 3 NIST ethanol-water standard reference solutions.

Keywords: kombucha, ethanol analysis, ionic liquid column, headspace gas chromatography



Country: USA

Citation: Food Anal. Methods (2017) 10:4062-4067 DOI 10.1007/s12161-017-0980-5

Study Mailing Address:
Department of Chemistry and Biochemistry, the University of Texas at Arlington, Arlington TX, USA

Date Updated: January 14, 2021