Fermented black tea ameliorates gamma radiationinducedcellular and DNA damage in human blood lymphocytes

Authors:
Tanmoy Mondal, Sandip Pal, Subrata Kumar Dey

Abstract:
This study was undertaken to evaluate the antioxidative potential and radioprotective ability Of fermented black tea (FBT). Total polyphenol content in the FBT was found to be 148.30 2.56 mg/g, as determined by the ferrous tartrate method. DPPH (2, 2-diphenyl-1-picryl hydrazyl) radical scavenging reached a maximum of 96% at 200 g/ml of FBT, and accordingly, the reducing power also saturated at the very same concentration. Studies carried out with plasmid DNA revealed the ability of FBT to inhibit gamma radiation-induced single as well as double-strand breaks. Prior administration of FBT to human lymphocytes significantly countered radiation-induced DNA damage as evaluated by micronuclei assay and chromosomal aberration study and cell death by trypan blue exclusion method. The results clearly indicate that FBT has significant potential to protect the cellular system from radiation-induced damage and the ability to scavenge free radicals might be playing an important role in its radioprotective manifestations


Keywords: radioprotection, fermented black tea, antioxidant, free radical, cbmn assay



Country: India

Citation: International Journal of Biotechnology Research Vol. 3(4), pp. 055-064, June

Study Mailing Address:
Department of Biotechnology, West Bengal University of Technology, Kolkata, West Bengal, India

Date Updated: March 9, 2020