Growth inhibition and induction of apoptosis in human bladder cancer cells induced by fermented citrus Kombucha



Authors:
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Abstract:
Kombucha is a slightly sour beverage fermented by symbiotic micro-organisms, including bacteria and yeasts. In this study, we examined the biological activities of citrus Kombucha (CK) produced by the addition of citrus extract to the original Kombucha (K). After fermentation for 10 days, radical scavenging activity examined by ABTS and DPPH assays increased by approximately 20% compared to that of K. Moreover, the content of total phenolic compounds significantly increased by 60% compared to that of K. Cell proliferation assays utilizing MTT showed that CK treatment significantly inhibited the growth of bladder cancer cells, T-24 and 5637, in a dose-dependent manner with IC50 values of 4 and 7 mg/mL, respectively. Annexin V staining showed that CK treatment led to apoptosis of cells in a dose-dependent manner. T-24 cells were more sensitive to CK treatment than 5637 cells, as 8 mg/mL of CK resulted in 97% apoptosis of T-24 cells. Western blotting showed that CK treatment led to the up-regulation of apoptotic proteins, including caspases-3, -8, -9, and PARP, in bladder cells not in K-treated cells. Taken together, these results demonstrate that CK may be developed as a functional beverage.

Keywords: cancer, cells, ph, protein



Country: Korea

Citation: J Korean Soc Food Sci Nutr 45(10), 14221429

Study Mailing Address:
Sung-Soo Park, Department of Food Science
& Nutrition, Jeju National University, Jeju-si, Jeju-do 63243, Korea

Date Updated: January 12, 2021

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