Novel nitrogen-fixing Acetobacter nitrogenifigenssp. nov., isolated from Kombucha tea

Authors:
Debasree Dutta, Ratan Gachhui

Abstract:
The four nitrogen-fixing bacteria so far described in the family Acetobacteraceae belong to the genera Gluconacetobacter and Acetobacter. Nitrogen-fixing bacterial strain RG1T was isolated
from Kombucha tea and, based on the phylogenetic analysis of 16S RRNA gene sequence which is supported by a high bootstrap value, was found to belong to the genus Acetobacter. Strain RG1T differed from Acetobacter aceti, the nearest member with a 16S RRNA gene sequence similarity of 98.2%, and type strains of other Acetobacter species with regard to several characteristics of growth features in culture media, growth in nitrogen-free medium, production of c-pyrone from glucose and dihydroxyacetone from glycerol. Strain RG1T utilized maltose, glycerol, sorbitol, fructose, galactose, arabinose, and ethanol, but not methanol as a carbon source. These results, along with electrophoretic mobility patterns of nine metabolic enzymes, suggest that strain RG1T represents a novel nitrogen-fixing species. The ubiquinone present was Q-9 and DNA G+C content was 64.1 mol%. Strain RG1T exhibited a low value of 2-24% DNA-DNA relatedness to the type strains of related acetobacters, which placed it as a separate taxon. On the basis of this data, the name Acetobacter nitrogenifigens sp. nov. is proposed, with the type strain RG1T (=MTCC 6912T=LMG 23498T).

Keywords: acetobacter, ethanol, fructose, gluconacetobacter, glucose, ph



Country: India

Citation: International Journal of Systematic and Evolutionary Microbiology, 56(8), 1899-1903.

Study Mailing Address:
Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700032, West

Date Updated: January 21, 2021