Optimizing Glucuronic Acid Production Using Tea Fungus on Grape Juice by Response Surface Methodology

Authors:
Nafiseh Yavari, Mahnaz Mazaheri Assadi, Mohammad Bamani Moghadam, Kambiz Larijani

Abstract:
Grape juice phytochemicals such as resveratrol and polyphenol antioxidants have been positively linked to inhibiting cancer heart disease degenerative nerve disease viral infections and mechanisms of Alzheimer's disease. Glucuronic acid is the key component in human health due to its detoxifying action through conjugation to the xenobiotic metabolisms in the liver and associated with cartilage, shown substantial benefit in the treatment of osteoarthritis. Here we report the first analysis of evaluate the effect of treatment variables sucrose content temperature and cultivation time on glucuronic acid production (g/L) as well as monitored changes in pH, remained sucrose (g/L), reducing sugar (g/L) and total acidity (g/L) by sing Kombucha layer on sweetened grape juice. Kombucha is a refreshing beverage obtained through the fermentation of sugared grape juice with a symbiotic culture of acetic bacteria and fungi consumed for its distinct antibiotic effects against the number of disease organisms and several therapeutic purposes in human medicine. Response surface methodology using Box-Behnken design showed that all the factors had a significant effect on glucuronic acid production. The optimum medium composition for predicted maximum glucuronic acid production was appeared on 7% sucrose-sweetened grape juice within two weeks of fermentation process at 37C.


Keywords: kombucha, sweetened grape juice, glucuronic acid, response surface methodology, therapeutic purposes



Country: Iran

Citation: Aust J Basic Appl Sci, 5, 1788-94.

Study Mailing Address:
Young Researchers club, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2
Biotechnology Department, Iranian Research Organization for Science and Technology.
3
Associated Professor, Department of Statistics, Alameh Tabatabaei University, Tehran, Iran.
4
Laboratory Complex, Islamic Azad University, Science and Research Branch, Tehran, Iran.

Date Updated: March 12, 2020