Screening the optimal ratio of symbiosis between isolated yeast and acetic acid bacteria strain from traditional kombucha for high-level production of glucuronic acid



Authors:
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Abstract:
Glucuronic acid-a a human detoxifying drug that can be found in traditional kombucha, which is a sweetened-black tea fermented by symbiotic microflora between yeast and acetic acid bacteria embedded within a microbial cellulose membrane. The main purpose of the study is to obtain the newly designed symbiosis from the isolated yeasts and bacterial strains, which can produce the high-level glucuronic acid kombucha and avoid unexpected microbial contaminants. The isolation, selection, and identification showed the best initial combination ratio between Dekkera bruxellensis KN89 and Gluconacetobacter intermedius KN89 is 4Y (yeast):6A (acetic acid -bacteria) in number of living cell per milliliter which produced 175.8 mg L1 glucuronic acid in 7-day fermentation (P < 0.05). This study also provides a basic understanding of fermentation kinetics of this symbiosis in order to control and enhance the final product at the critical time point (after 54 hrs of process). The findings of this study are practically relevant in producing a safe and glucuronic acid enriched kombucha.

Keywords: combination ratios, fermented tea, glucuronic acid, kombucha, sweetened-black tea



Country: Viet Nam

Citation: LWT-Food Science and Technology, 64(2), 1149-1155.

Study Mailing Address:
School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, 70000, Viet Nam
b Center of Research and Technology Transfer, International University, Vietnam National University, Ho Chi Minh City, 70000, Viet Nam
c Department of Biotechnology, Faculty of Chemical Engineering, University of Technology, Vietnam National University, Ho Chi Minh City, 70000, Viet Nam

Date Updated: January 26, 2021

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