The frequency of sister chromatid exchange and micronuclei in evaluation of cytogenetic activity of Kombucha on human peripheral blood lymphocytes
Jasminka Mrdanovic, Gordana Bogdanovic, Dragoljub D Cvetkovic, Aleksandra S Velicanski, Dragana D Cetojevic-Simin
Kombucha is a refreshing beverage obtained by the fermentation of sweetened black tea with a "tea fungus" (symbiotic culture of acetic acid bacteria and yeasts). It is consumed due to its potential beneficial effects on human health. The aim of this study was to investigate the activity of Kombucha on human peripheral blood lymphocytes in vitro. We analyzed Kombucha made from different substrates: Camellia sinensis and Satureja montana, and effects of substrates alone. The frequencies of sister chromatid exchange (SCE) and micronuclei (MN) were scored as genetic endpoints, and mitomycin C was used as a model mutagen. Kombucha from camellia sinensis and camellia sinensis substrate increased frequency of MN and SCE on mitomycin C-treated and -untreated peripheral blood lymphocytes. However, Kombucha from Satureja montana reduced the incidence of MN on mitomycin C-treated and -untreated peripheral blood lymphocytes, while SCE frequency was higher than the control value. In our pilot study, we showed for the first time that Kombucha from different substrates induced different effects on mitomycin C-treated and -untreated peripheral blood lymphocytes.
Keywords: lymphocytes, cells, cultured, beverages, tea, yeasts, sister chromatid exchange, micronuclei, chromosome-defective, mitomycin
Citation: Arch Oncol 2007;15(3-4):85-8.
Study Mailing Address:
1 Oncology Institute of Vojvodina, Sremska Kamenica, Serbia, 2 Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Novi Sad, Serbia
Date Updated: February 16, 2021